Current Protocols in Cytometry

Current Protocols in Cytometry is the comprehensive resource for all types of protocols in cytometry and imaging. The expansion of cell-based analysis systems across all fields of biology has been driven by the demand for high-content information integrated across proteomics and genomics. Cytometry covers a wide array of technologies, all of which are complex and demand attention to assay design, data collection, and multivariate analysis. Current Protocols in Cytometry includes protocols for basic, clinical and translational applications and features step-by-step instructions for performing hundreds of complex assays utilizing tracking probes, multiple reporter molecules, and analyzing big data from high-throughput cytometric and imaging analysis.. Vital information such as safety issues for highly dangerous pathogens, statistical considerations and instrumentation is covered in detail. The publication covers approaches ranging from single-cell analysis using flow cytometry to large-scale imaging using high-content screening and analysis. An extensive section of the publication covers advanced data analysis integrating current techniques of classification and automated identification. Coverage includes:

Flow Cytometry Instrumentation;
Image Cytometry Instrumentation;
Safety Procedures and Quality Control;
Molecular and Cellular Probes;
Specimen Handling, Storage, and Preparation;
Phenotypic Analysis;
Nucleic Acid Analysis;
Molecular Cytogenetics;
Studies of Cell Function;
Data Processing and Analysis;
Microbiological Applications;
Cellular and Molecular Imaging;
Multiplexed and Microparticle-Based Analyses.